Journal: Advances in Wound Care

Article Title: Skin Microcolumns as a Source of Paracrine Signaling Factors

doi: 10.1089/wound.2019.1045

Figure Lengend Snippet: MSTC-conditioned media induced endothelial tube formation by HUVECs, but control media did not ( A , each image width corresponds to 1.2 mm). Number of junctions (B) , total tube length (C) , and average tube length (D) were all significantly increased in response to the MSTC-conditioned media. Mean ± 95% CI. * p < 0.05, ** p < 0.005, n = 4. HUVEC, human umbilical vein endothelial cell. Color images are available online.

Article Snippet: Human umbilical vein endothelial cells (HUVECs, purchased from ATCC) were cultured and maintained in Medium 200PRF supplemented with low serum growth supplement (Thermo Fisher Scientific).

Techniques: Control

Journal: Advances in Wound Care

Article Title: Skin Microcolumns as a Source of Paracrine Signaling Factors

doi: 10.1089/wound.2019.1045

Figure Lengend Snippet: Partial list of soluble proteins released by micro skin tissue columns that are known to significantly impact the wound healing process

Article Snippet: Human umbilical vein endothelial cells (HUVECs, purchased from ATCC) were cultured and maintained in Medium 200PRF supplemented with low serum growth supplement (Thermo Fisher Scientific).

Techniques: Migration

Journal: Microsystems & Nanoengineering

Article Title: Modular operation of microfluidic chips for highly parallelized cell culture and liquid dosing via a fluidic circuit board

doi: 10.1038/s41378-020-00216-z

Figure Lengend Snippet: a Live-cell fluorescence images (one image per four chambers) showing an overview of all the chambers after 3 days of culturing GFP-expressing HUVECs. b Live-cell fluorescence image of GFP-expressing HUVECs in chambers 6 and 7. c Fluorescence image of fixed HUVECs with the cell F-actin and nuclei stained with ActinRed and NucBlue, respectively. d HUVECs after seeding and subsequent monolayer formation. The cells were seeded at a high cell density (i) and confluent on day 1 (ii). The monolayer was still intact on day 3 (iii) but began to deteriorate on day 4 (iv). At this point, the medium in the supply vial was replaced. The cells showed signs of recovery on day 5 (v) as the monolayer started to reform. The red dots in (iv) and (v) mark the cells counted in the region of interest. e Cells in ten chambers were counted in regions where the monolayer had deteriorated on day 4 (98 h) and after 16 h of recovery (114 h). The black dots represent the mean cell count, and the error bars represent the standard deviation. The cell number increased by an average of 33% in these areas

Article Snippet: Live-cell images of the GFP-expressing HUVECs (Angio-Proteomie, USA) were taken with an EVOS FL cell imaging system using the GFP filter cube.

Techniques: Fluorescence, Expressing, Staining, Cell Counting, Standard Deviation